Novum SLE Information

Simplify Your Liquid-Liquid Extractions

Novum Simplified Liquid Extraction (SLE) (patent pending) products are a FASTER, EASIER, and MORE RELIABLE way to perform liquid-liquid extractions. In 3 easy steps and less than 15 minutes, you can remove unwanted interferences such as salts, proteins, phospholipids and more from a variety of samples without performing extensive method development. Available in several capacities, Novum SLE is an excellent cleanup solution for a wide range of samples including biological, food, personal care products, and more.

As the first synthetic SLE product on the market, Novum provides consistent, reliable results from lot-to-lot which is sometimes difficult to do when using a natural product such as traditional diatomaceous earth SLE.

Get Down to the Lowest Extraction Levels with Novum PRO SLE

Offers the same reliable synthetic sorbent as Novum with additional clean

manufacturing steps to reach low levels of detection for sensitive MS applications,

with the same quality reproducibility for high-throughput samples.

• Specific manufacture capabilities to improve matrix factor response and reduce

noisy baselines for low level testing of biological samples

• API 6500+ fit for purpose testing to ensure clean baseline with each batch

• Available in both MINI and MAX 96-well plate formats for high-throughput

applications

Novum PRO MAX 96-well Plate Part No,: 8E-S539-5GA

Novum PRO MINI 96-well Plate Part No,: 8E-S539-FGA

View the brochure here>>

A Variety of Formats to Fit Your Sample Requirements

SLE Tubes




Novum SLE Tube	1cc	3cc	6cc	12cc
Maximum Sample Volume (before dilution)	100µL	200µL	500µL	1mL
Recommended Elution Volume	1.2mL	1.8mL	5mL	10mL

96 Well Plates




Novum SLE 96-Well Plates	MIN	MAX
Maximum Sample Volume (before dilution)	150µL	200µL
Recommended Elution Volume	1mL	1.8mL

Check out our publications

Don't forget the accessories!

Sample Preparation Accessories Guide

Sample Processing to Laboratory Safety

Vacuum Manifolds
Collection Plates
Verex™ Vials
Laboratory Safety
And more...

Download the Sample Prep Accessories Guide Now »

FAQs»

Novum liquid extraction spe sample preparation

See What Makes Novum Different

<div class="nov-main">
<p>Novum Simplified Liquid Extraction (SLE) (patent pending) products are a <strong>FASTER</strong>, <strong>EASIER</strong>, and <strong>MORE RELIABLE </strong>way to perform liquid-liquid extractions.  In 3 easy steps and less than 15 minutes, you can remove unwanted interferences such as salts, proteins, <a href="http://www.phenomenex.com/Application/Detail/22730?returnURL=/Search">phospholipids</a> and more from a variety of samples without performing extensive method development. Available in several capacities, Novum SLE is an excellent cleanup solution for a wide range of samples including biological, food, personal care products, and more.</p>

<p>As the first synthetic SLE product on the market, Novum provides consistent, reliable results from lot-to-lot which is sometimes difficult to do when using a natural product such as traditional diatomaceous earth SLE.</p>

<h3><big>Get Down to the Lowest Extraction Levels with Novum PRO SLE</big></h3>

<div><span style="font-size: 13px;">Offers the same reliable synthetic sorbent as Novum with additional clean</span></div>

<div><span style="font-size: 13px;">manufacturing steps to reach low levels of detection for sensitive MS applications,</span></div>

<div><span style="font-size: 13px;">with the same quality reproducibility for high-throughput samples.</span></div>

<div><span style="font-size: 13px;">• </span>Specific manufacture capabilities to improve matrix factor response and reduce</div>

<div><span style="font-size: 13px;">noisy baselines for low level testing of biological samples</span></div>

<div><span style="font-size: 13px;">• API 6500+ fit for purpose testing to ensure clean baseline with each batch</span></div>

<div><span style="font-size: 13px;">• Available in both MINI and MAX 96-well plate formats for high-throughput</span></div>

<div><span style="font-size: 13px;">applications</span></div>

<div>Novum PRO MAX 96-well Plate Part No,: 8E-S539-5GA</div>

<div>Novum PRO MINI 96-well Plate Part No,: 8E-S539-FGA</div>

<p><a href="https://www.phenomenex.com/ViewDocument?id=simply+your+sle+with+novum">View the brochure here>></a></p>

<h2 style="margin-top: 15px;">A Variety of Formats to Fit Your Sample Requirements</h2>

<p id="newtubes" style="font-size: 14px; font-weight: bold;">SLE Tubes</p>

<table id="tubechart">
	<tbody>
		<tr>
			<td colspan="5"> </td>
		</tr>
		<tr>
			<td> </td>
			<td valign="bottom"><img alt="Novum SLE tube 1cc" src="/webimage/sle-tube-sm.png" style="padding-left: 15px;" title="Novum SLE tube 1cc" width="25" /></td>
			<td valign="bottom"><img alt="Novum SLE tube 3cc" src="/webimage/sle-tube-sm.png" style="padding-left: 15px;" title="Novum SLE tube 3cc" width="30" /></td>
			<td valign="bottom"><img alt="Novum SLE tube 6cc" src="/webimage/sle-tube-sm.png" style="padding-left: 15px;" title="Novum SLE tube 6cc" width="35" /></td>
			<td valign="bottom"><img alt="Novum SLE tube 12cc" src="/webimage/sle-tube-sm.png" style="padding-left: 15px;" title="Novum SLE tube 12cc" width="45" /></td>
		</tr>
		<tr>
			<td colspan="5" height="8"> </td>
		</tr>
		<tr>
			<td style="padding: 7px 5px 5px; color: rgb(255, 255, 255); font-weight: bold; background-color: rgb(123, 177, 216);" width="35%">Novum SLE Tube</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">1cc</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">3cc</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">6cc</td>
			<td style="text-align: center;">12cc</td>
		</tr>
		<tr>
			<td style="padding: 7px 5px 5px; color: rgb(255, 255, 255); font-weight: bold; background-color: rgb(0, 129, 188);">Maximum Sample Volume<br />
			(before dilution)</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid; background-color: rgb(225, 234, 244);">100µL</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid; background-color: rgb(225, 234, 244);">200µL</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid; background-color: rgb(225, 234, 244);">500µL</td>
			<td style="text-align: center; background-color: rgb(225, 234, 244);">1mL</td>
		</tr>
		<tr>
			<td style="padding: 7px 5px 5px; color: rgb(255, 255, 255); font-weight: bold; background-color: rgb(123, 177, 216);">Recommended Elution Volume</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">1.2mL</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">1.8mL</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">5mL</td>
			<td style="text-align: center;">10mL</td>
		</tr>
		<tr>
			<td colspan="5" height="20"> </td>
		</tr>
	</tbody>
</table>

<p style="font-size: 14px; font-weight: bold; margin-top: 20px;">96 Well Plates</p>

<table id="96wellplateschart">
	<tbody>
		<tr>
			<td colspan="5" height="9"> </td>
		</tr>
		<tr>
			<td> </td>
			<td><img alt="Novum SlE Well Plates 150" src="/webimage/sle-novum_wp-sm.png" style="padding-left: 10px;" width="125" /></td>
			<td><img alt="Novum SlE Well plates 200" src="/webimage/sle-novum_wp-sm.png" style="padding-left: 10px;" width="125" /></td>
		</tr>
		<tr>
			<td colspan="5" height="8"> </td>
		</tr>
		<tr>
			<td style="padding: 7px 5px 5px; color: rgb(255, 255, 255); font-weight: bold; background-color: rgb(123, 177, 216);" width="35%">Novum SLE 96-Well Plates</td>
			<td style="text-align: center; font-weight: bold; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">MIN</td>
			<td style="text-align: center; font-weight: bold;">MAX</td>
		</tr>
		<tr>
			<td style="padding: 7px 5px 5px; color: rgb(255, 255, 255); font-weight: bold; background-color: rgb(0, 129, 188);">Maximum Sample Volume<br />
			(before dilution)</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid; background-color: rgb(225, 234, 244);">150µL</td>
			<td style="text-align: center; background-color: rgb(225, 234, 244);">200µL</td>
		</tr>
		<tr>
			<td style="padding: 7px 5px 5px; color: rgb(255, 255, 255); font-weight: bold; background-color: rgb(123, 177, 216);">Recommended Elution Volume</td>
			<td style="text-align: center; border-right-color: rgb(0, 129, 188); border-right-width: 1px; border-right-style: solid;">1mL</td>
			<td style="text-align: center;">1.8mL</td>
		</tr>
		<tr>
			<td colspan="5" height="20"> </td>
		</tr>
	</tbody>
</table>

<h2 style="margin-bottom: 0px;">Check out our publications</h2>

<ul style="margin-top: -1px;">
	<li style="padding-top: 5px;"><a href="http://www.americanlaboratory.com/914-Application-Notes/172516-A-Simplified-Approach-to-Bioanalytical-Sample-Preparation/" target="_blank">A Simplified Approach to Bioanalytical Sample Preparation</a></li>
	<li style="padding-top: 5px;"><a href="http://www.mlo-online.com/articles/201501/achieving-excellent-drugs-of-abuse-analysis-starts-with-the-proper-sample-preparation-technique.php" target="_blank">Achieving Excellent Drugs-of-Abuse Analysis Starts with the Proper Sample Preparation Technique</a></li>
	<li style="padding-top: 5px;"><a href="http://images2.advanstar.com/PixelMags/lctc/digitaledition/February19-2015-us.html#18" target="_blank">Simplifying Liquid Extractions</a></li>
	<li style="padding-top: 5px;"><a href="http://www.foodqualityandsafety.com/article/a-timesaving-approach-to-sample-prep/" target="_blank">A Time Saving Approach to Sample Prep</a></li>
</ul>

<hr />
<h2 style="margin-bottom: 0px;">Don't forget the accessories!</h2>
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<div class="indus">
<p> </p>

<ul class="spacclist">
</ul>
<strong>Sample Preparation Accessories Guide</strong>

<table>
	<tbody>
		<tr>
			<td> </td>
			<td>Sample Processing to Laboratory Safety
			<ul class="spaccbenefit">
				<li>Vacuum Manifolds</li>
				<li>Collection Plates</li>
				<li>Verex™ Vials</li>
				<li>Laboratory Safety</li>
				<li>And more...</li>
			</ul>
			<a class="btn btn-primary btn-small" href="https://phenomenex.blob.core.windows.net/documents/38514ae6-a5ff-4184-a739-6bf4708728f9.pdf" style="margin-top:5px;margin-left:20px">Download the Sample Prep Accessories Guide Now »</a></td>
		</tr>
	</tbody>
</table>
</div>

<h2 id="novumvid" style="margin-bottom: 0px;">See What Makes Novum Different</h2>

<h1>Novum FAQS</h1>
</div>

<div class="modal-body;" style="height: 575px;">
<div class="mg-faq">
<p><span class="novum-q">Moving a Liquid-Liquid Extraction to Simplified Liquid Extraction (SLE)</span></p>

<p>If you are already performing a liquid-liquid extraction, you may use the same buffers and solvents you are using in your liquid-liquid extraction to perform SLE.</p>

<p class="nofaq"><span class="novum-q">Elution Solvent Selection</span></p>

<p>In most cases, using ethyl acetate as an elution solvent results in an excellent balance between cleanup and recovery.</p>

<p class="nofaq">If you are using DCM as an elution solvent and see low recovery of target analytes (particularly fluorinated analytes or those that have a negative logP value), recovery may be improved by adding 10-20 % Ethyl acetate to the DCM during the elution step.</p>

<p class="nofaq"><span class="novum-q">Elution Volumes</span></p>

<p>If desired, you may apply multiple aliquots of elution solvent. For example, 3x 600 µL of elution solvent may be used instead of 2x 900 µµL on the Novum SLE MAX 96-well plate.</p>

<p class="nofaq"><span class="novum-q">Boosting Analyte Recovery</span></p>

<p>The Novum SLE sorbent relies on mass transfer to help partition target analytes out of the aqueous sample and into the organic elution solvent. In order to boost recoveries of some assays, it may be necessary to dilute your sample more than the suggested 1:1 dilution in order to increase the surface area of the aqueous sample. See Recommended Loading Volumes in the General Protocol to determine the total load volume (sample plus diluent) for each Novum SLE product.</p>

<p>It may also be possible to boost analyte recoveries by increasing the amount of elution solvent applied to the sorbent.</p>

<p>Longer elution times will also improve extraction efficiencies in many cases. If vacuum must be applied, it is recommended that the vacuum pressure applied results in drop wise elution of the extraction solvent.</p>
<img src="/webimage/novum-logo-shot.png" style="margin-top: 10px; margin-left: 55px;" /></div>
</div>
</div>
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Featured

TECHNICAL NOTE

Liquid-Liquid Extraction vs. Simplified Liquid Extraction

A case study to look into the benefits of Novum SLE compared to traditional LLE.
BROCHURE

A NEW Way to Simplify Your Liquid Extractions

Skip the shaking and start saving time while generating reproducibly clean samples.
VIDEO

Balancing Cleanup, Cost, and Time

This on demand webinar takes a look at sample preparation techniques and downstream HPLC/UHPLC optimization to overcome challenging matrices.
TECHNICAL NOTE

Extraction of Parabens Using Novum Simplified Liquid Extraction (SLE)

Using Novum SLE to extract parabens from baby wash and quantifying based on LC/UV.

Technical Documents
Search FAQ

Document Type:

Document Type	Title
Supporting Docs	Novum SLE Tutorial Video	Add to My Documents
Webinar	On Demand Webinar: A New Way to Simplify Your Liquid Extractions	Add to My Documents
Supporting Docs	See What Makes Novum Simplified Liquid Extraction (SLE) Products Different	Add to My Documents
Technical Notes	Acrylamide from Coffee (TN-0079)	Add to My Documents
Technical Notes	Automated Cleanup of Cortisone and Prednisolone from Plasma (TN-0077)	Add to My Documents
Technical Notes	Drugs of Abuse from Urine using Novum SLE	Add to My Documents
Technical Notes	Liquid-Liquid Extraction (LLE) vs. Novum Simplified Liquid Extraction (SLE) Case Study: Analysis of Monomeric Phenolic Fermentation Inhibitors in Dilute-Acid Plant Hydrolysis	Add to My Documents
Technical Notes	NSAIDs from Plasma using Novum Simplified Liquid Extraction (SLE)	Add to My Documents
Technical Notes	Parabens from Baby Wash using Novum SLE and a Kinetex EVO C18 HPLC Column (TN-0081)	Add to My Documents
Technical Notes	THC-COOH from Urine using Novum SLE	Add to My Documents
Technical Notes	Total Cortisol from Plasma using Novum SLE (TN-0076)	Add to My Documents
Technical Notes	Vitamin D2 and D3 from Serum using Novum SLE	Add to My Documents
Other	On-Demand Webinar: Novum Simplified Liquid Extraction (SLE) 96-Well Plates	Add to My Documents
Supporting Docs	Novum Simplified Liquid Extraction (SLE) User Guide	Add to My Documents

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Faster, Easier and More Reliable

than Liquid-Liquid Extraction.

Novum SLE Information

Simplify Your Liquid-Liquid Extractions

Get Down to the Lowest Extraction Levels with Novum PRO SLE

A Variety of Formats to Fit Your Sample Requirements

Check out our publications

Don't forget the accessories!

See What Makes Novum Different

Featured

Liquid-Liquid Extraction vs. Simplified Liquid Extraction

A NEW Way to Simplify Your Liquid Extractions

Balancing Cleanup, Cost, and Time

Extraction of Parabens Using Novum Simplified Liquid Extraction (SLE)