Phase	Description	Application
Silica(2)	Unbonded silica	Non-polar compounds
C5	5 Carbon ligand	Good alternative to C8 when less retention is desired
C8(2)	C8 ligand optimized for improved peak shape	Great starting phase for method development
C18(2)	C18 ligand optimized for improved peak shape	From capillary LC/MS to process scale Our most popular phase
CN	Versatile CN phase	For improving the retention of polar compounds
NH₂	Rugged and reproducible NH	Sugar alcohols, anionic or hydrogen bonding compounds
Phenyl-Hexyl	Phenyl phase attached to C6 (hexyl) ligand	Unique selectivity for very polar and aromatic compounds
SCX	Benzene sulfonic acid	Amine and polyamine containing compounds
HILIC	Reproducible, cross-linked diol	Increased retention and MS sensitivity of polar compounds
PFP(2)	Pentafluorophenyl with a C3 (propyl) linkage	Highly polar compounds, halogenated compounds and isomers

Particle Sizes (µm)
Pore Size (Å)
Surface Area (sq. m/g)
Carbon Load (%)

Compare All Phases

Phase Information

}

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		background-color:#fff;
	color:#000;
	padding-left: 10px;
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<div id="lunac18">
<h3>Excellent Polar Selectivity</h3>

<p>Luna HILIC phase has a silica surface that is covered with cross-linked diol groups for polar selectivity under hydrophilic liquid chromatography conditions . The Luna HILIC HPLC column provides excellent selectivity for polar compounds and offers MS sensitivty with low bleed.</p>

<div class="mrow">
<div class="mcol-50">
<table class="lunared">
	<tbody>
		<tr>
			<td class="redlane" valign="top">Phase<br />
			Description</td>
			<td class="graylane">Cross-linked diol that provides excellent reproducibility</td>
		</tr>
		<tr>
			<td class="redlane" valign="top">USP<br />
			Classification</td>
			<td class="whitelane">L20:Dihydroxypropane groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter</td>
		</tr>
		<tr>
			<td class="redlane" valign="top">Trending<br />
			Applications</td>
			<td class="graylane">
			<div class="mrow">
			<ul class="luna-apps m-one" style="margin-left:-25px;padding-right:10px">
				<li><a href="/Application/Detail/19004" target="_blank">Atenolol</a></li>
				<li><a href="/Application/Detail/16389" target="_blank">Ciprofloxacin</a></li>
				<li><a href="/Application/Detail/18999" target="_blank">Metaprolol </a></li>
			</ul>

<ul class="luna-apps m-two">
				<li><a href="/Application/Detail/16334" target="_blank">Morphine</a></li>
				<li><a href="/Application/Detail/18997" target="_blank">Propafenone</a></li>
				<li><a href="/Application/Detail/18991" target="_blank">Terfendadine</a></li>
			</ul>
			</div>
			</td>
		</tr>
		
	</tbody>
</table>
</div>

<div class="mrow">
<h3>Popular Part Numbers</h3>

<table class="luna-parts">
	<tbody>
		<tr>
			<th>Part Number</th>
			<th>Particle Size (µm)</th>
			<th>Pore Size (Å)</th>
			<th>Length (mm)</th>
			<th>I.D. (mm)</th>
		</tr>
		<tr>
			<td><a href="/products/part/00d-4449-b0">00D-4449-B0</a></td>
			<td>3 µm</td>
			<td>200 Å</td>
			<td>100</td>
			<td>2</td>
		</tr>
		<tr>
			<td><a href="/products/part/00f-4449-b0">00F-4449-B0</a></td>
			<td>3 µm</td>
			<td>200 Å</td>
			<td>150</td>
			<td>2</td>
		</tr>
		<tr>
			<td><a href="/products/part/00f-4449-e0">00F-4449-E0</a></td>
			<td>3 µm</td>
			<td>200 Å</td>
			<td>150</td>
			<td>4.6</td>
		</tr>
		<tr>
			<td><a href="/products/part/00F-4449-e0">00F-4450-E0</a></td>
			<td>5 µm</td>
			<td>200 Å</td>
			<td>150</td>
			<td>4.6</td>
		</tr>
		
	</tbody>
</table>
<a class="btn btn-primary btn-small" href="/Products/HPLCDetail/luna?order=true" style="margin-top:10px">View all Luna HPLC* column part numbers »</a>  </div>

<div class="mrow">
<h3>Frequently Asked Questions</h3>

<ul class="luna-questions" style="margin-top:-10px">
	<li><a href="/FAQ/Details/1dd84a61-d8e9-49bf-a336-3739429f299d/what-solvents-can-be-used-as-a-mobile-phase-under-hilic-conditions/?lang=en" target="_blank">What solvents can be used as a mobile phase under HILIC conditions?</a></li>
	<li><a href="/FAQ/Details/3a8d1a1b-8399-4a19-8299-20ada4af1e94/what-are-some-general-guidelines-before-beginning-method-development-on-my-hilic/?lang=en" target="_blank">What are some general guidelines before beginning method development on my HILIC method?</a></li>
	<li><a href="/FAQ/Details/6ef83799-b4ca-4839-96b4-2ab720e4b250/how-will-the-sample-diluent-affect-hilic-separations/?lang=en" target="_blank">How will the sample diluent affect HILIC separations?</a></li>
</ul>
<img src="/webimage/premium-column-angle-silver.png" style="margin-top:5px; margin-left:20px;" width="150" /> </div>

<div class="col-70">
<h3>HILIC LC Column Care & Use</h3>

<ul class="luna-questions" style="margin-top:-5px;">
	<li>Column Flow Rate and Backpressure</li>
	<li>Column Installation</li>
	<li>Mobile Phase Compatibility</li>
	<li>Shipping Solvent</li>
</ul>
<a class="btn btn-primary btn-small" href="http://az621941.vo.msecnd.net/documents/835c6666-c915-47f3-bbcc-cd413a9bdedc.pdf" style="margin-left:20px;" target="_blank">Download Now »</a> </div>
</div>
</div>

23 Phases

Particle Size (µm):	ID (mm):
1.6	1.0 (Microbore) 2.1 (Minibore)
3	2.1 (Minibore) 3.0 (MidBore) 4.6 (Analytical)
5	2.1 (Minibore) 3.0 (MidBore) 4.6 (Analytical) 21.2 (Prep

Particle Size (µm):	ID (mm):
1.6	1.0 (Microbore) 2.1 (Minibore)

3	0.3 (Micro Flow) 0.5 (Micro Flow) 2.1 (Minibore) 3.0 (MidBore™) 4.6 (Analytical)

5	0.3 (Micro Flow) 0.5 (Micro Flow) 2.1 (Minibore) 3.0 (MidBore™) 4.6 (Analytical) 10 (Semi-Prep) 21.2-50 (Prep)

Particle Size (µm):	ID (mm):
1.6	1.0 (Microbore) 2.1 (Minibore)

3	0.3 (Micro Flow) 0.5 (Micro Flow) 2.1 (Minibore) 3.0 (MidBore™) 4.6 (Analytical)

5	0.3 (Micro Flow) 0.5 (Micro Flow) 2.1 (Minibore) 3.0 (MidBore™) 4.6 (Analytical) 10 (Semi-Prep) 21.2-50 (Prep)

Phase	Particle Sizes (µm)	Pore Size (Å)	Surface Area (sq. m/g)	Carbon Load (%)	Recommended Use
C18	3, 5, 10	100	440	19	Separation of hydrophobic compounds
C18(2)	2.5, 3, 5, 10, 15	100	400	17.5	Separation of hydrophobic compounds
C5	5, 10	100	440	12.5	Separation of extremely hydrophobic compounds
C8	3, 5, 10	100	440	14.75	Separation of very hydrophobic compounds
C8(2)	3, 5, 10, 15	100	400	13.5	Separation of very hydrophobic compounds
CN	3, 5, 10	100	400	7	Separation of polar compounds
HILIC	3, 5	200	200*	5.7	Separation of polar compounds
NH2	3, 5, 10	100	400	9.5	Separation of polar compounds
Omega C18	1.6, 3, 5	100	260	11.0
Omega Polar C18	1.6, 3, 5	100	260	11.0
Omega PS C18	1.6, 3, 5	100	260	11.0
Omega SUGAR	3	100		N/A
PFP(2)	3, 5	100	400	N/A	Separation of aromatic and halogenated compounds
Phenyl-Hexyl	3, 5, 10, 15	100	400	17.5	Separation of aromatic compounds
Polar Pesticides	3	100		N/A
PREP C18(2)	10	100		N/A
PREP C18(3)	10	100		N/A
PREP C4	10	100		N/A
PREP C8(2)	10	100		N/A
PREP C8(3)	10	100		N/A
PREP Phenyl-Hexyl	10	100		N/A
PREP Silica(2)	10	100		N/A
PREP Silica(3)	10	100		N/A
SCX	5, 10	100	400	0.55 % Sulfur Load	Separation of positively charged compounds
Silica (2)	3, 5, 10, 15	100	400	N/A	Separation of polar compounds

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Luna HPLC Information

^NEWLuna Omega C18 (1.6 μm, 3 μm, 5 μm)

Learn how the thermally modified fully porous architecture of the Luna Omega can improve your analysis:

Full Scalability

Real Reliability

Selective

The Technology Behind Luna Omega

Thermal Modified Pore Structure

Luna Omega C18

Luna Omega Polar C18

Luna Omega PS C18

See the Technology in Action

Luna HPLC and PREP

Featured

Extensive Selectivities of Luna HPLC Columns

NEW Luna Omega 1.6µm and 5µm LC Columns

Increasing LC/MS/MS Sensitivity with Luna HILIC

6 Reasons to Try Luna Omega Instead of ACQUITY® BEH

Phase Information

One of the World's

Leading HPLC Columns

Phase Information